Genotoxicity assessment of a pharmaceutical effluent using four bioassays
Robeck, J. Research was conducted to develop an effective method for cryopreserving bottlenose dolphin Tursiops truncatus semen processed immediately after collection or after h liquid storage. In each of two experiments, four ejaculates were collected from three males. Evaluations were conducted at collection, prefreeze, and 0-, 3-, and 6-h postthaw.
The use of cryopreserved dolphin spermatozoa facilitates the exchange of genetic material between aquatic parks and makes spermatozoa accessible to laboratories for studies to further our understanding of marine mammal reproduction. Heterologous IVF, a replacement for homologous IVF, could provide a means to test the sperm fertility potential; to study gamete physiology and early embryo development; and to avoid the use of valuable dolphin oocytes, which are difficult to obtain. Here, we present protocols that have been successfully used to collect and cryopreserve dolphin spermatozoa. The collection of semen is performed by manual stimulation on trained dolphins.
Combinations of laser scissors and tweezers can make it possible to perform subtle subcellular manipulations. In a procedure that should be feasible within a decade, two tweezers beams pink hold a cell firmly in place. One scissor beam lighter blue penetrates the cell to delete a faulty gene red. A second scissors beam dark blue cuts a hole in the cell membrane through which a competent genetic sequence black dots can pass. Clones of the genetically altered cell could be then produced and transplanted into the body for therapeutic use.